New Delhi, February 05: Scientists have discovered two actinomycetes bacterial strains for crop protection against a fungal plant pathogen called Colletotrichum gloeosporioides. The pathogen poses a major threat to large cardamom plants, which are a major source of income for the farmers of Sikkim. The lead scientists behind this discovery are from the Department of Biotechnology’s Imphal-based Institute of Bioresources and Sustainable Development (IBSD).
The strains called Streptomyces vinaceus RCS260 and Kitasatospora aburavienis RCS252 were discovered when researchers were investigating the antimicrobial potential of actinomycetes isolated from rhizosphere soil (soil near roots of plants) from Lachung area of northern Sikkim. Actinomycetes are a heterogeneous group of anaerobic Gram-positive bacteria well-known for filamentous and branching growth patterns. Encouraging results led to the extraction and analysis of metabolites produced by the actinomycetes.
The researchers isolated 26 actinomycetes from the soil samples. Of them, two strains, Streptomyces vinaceus RCS260 and Kitasatospora aburavienis RCS252 produced extracellular enzymes such as chitinase, cellulase, and protease along with other antimicrobial metabolites. They showed antimicrobial action against a wide range of plant pathogens which could be ascribed to action of hydrolytic enzymes including chitinase, alpha 1, 3-glucanase, chitosanase and protease.
Studies on actinomycetes isolated from different environments have attracted attentions of scientists and industries for the production of secondary metabolites and other functionalities. Actinomycetes isolated from extreme environments such as Antarctica and some habitats of Manipur and Sikkim have produced valuable metabolites of immense importance to industry and agriculture.
Sikkim is one of the largest producers of large cardamom. However, the state has reported significant decline in its yield over past few years. Among many factors responsible for the decline of crop yield, the fungal blight cause by Colletotrichum gloeosporioides is a major concern. (India Science Wire)